PeakSplitter is a Java GUI application for that will help you subdivide broad peaks, subdividing broad peaks into individual binding sites.

Usage: java -jar splitPeaks.jar <options>
Options include:
-help,-? displays help information
-p,--peakFile <string> input peak file
-w,--wigFile <string> input wig file or folder
-o,--outDir <string> output folder
-x,--prefix <string> string to add to output file names
-c,--cutoff <decimal integer> height cutoff (default 5)
-v,--valley <float> float value to determine the valley depth required for peak separation (default 0.6)
-f,--fetch <boolean> whether to fetch subpeaks sequences or not (default true)
-u,--url <string> Das url (default is for human "www.ensembl.org/das/Homo_sapiens.GRCh37.reference"
-n,--numSeq <decimal integer> number of best peak sequences to fetch (default 300)
-l,--length <decimal integer> length of sequence to fetch (default 60)

Requirements:
* Java

The license of this software is Freeware, you can free download and free use this calculator software.