FASTML is a software for computing the ancestral amino-acid sequences of a phylogenetic tree.

FASTML uses flags in the command line arguments:
-For help, type "fastml.exe -h"
-s seq.aln = the sequence input file name.
-t tree.txt = the input tree file name (Newick format).
The software automatically recognize any of these sequence formats: Phylip, Molphy, Fasta, Mase, Clustal, or Nexus.

The license of this software is Freeware, you can free download and free use this calculator software.