Cobra File Defrag is a useful utility that performs file based defragmentation. Most defragmentation tools work on an entire disk or individual drives. File Defrag is different as it allows you, using an Explorer style screen, to see defragmentation of every file and folder on each of your disks. Each fragmented file or folder can be individually defragmented.
It will also allow you to defragment what you want file by file or folder by folder rather than being forced to defragment a whole drive as with conventional tools.
File Defrag employs an innovative technique to reduce future fragmentation by moving file blocks into fragmented free space on the drive. This reduces the chances of new files themselves becoming fragmented and maximises the amount of contiguous free space on the drive.
Works with FAT, FAT32 and NTFS disk formats, as well as Windows compressed files.
Comes with comprehensive user guide

The license of this software is Free Trial Software, the price is $8, you can free download and get a free trial.