Analyze expression data with this utility. BCLUST is a program to investigate the reliability of gene clusters derived from largescale gene expression data by using hierarchical clustering algorithms. Because with the rapid development of microarray technologies, many of statistical procedures, most notably a variety of clustering algorithms, have been successfully applied to analyze such kind of data. But in essentially all published studies, the observed gene expression levels are treated as if they were an accurate measure of the true expression level, and effects of variations and uncertainties in measured gene expression levels across samples and experiments have been seldom addressed. In the context of hierarchical clustering algorithms, we introduce a statistical resampling method to assess the reliability of gene clusters identified from any hierarchical clustering method. Using the clustering trees constructed from the resampled data sets, we can evaluate the confidence value for each node in the observed clustering tree. A majority-rule consensus tree can be obtained showing clustering that only occur in a majority of resampled trees.

The license of this software is Freeware, you can free download and free use this calculator software.