Calculate Markov matrices with this tool. 2STM calculates Markov matrices from 2-state character data sets with 3 sequences simultaneously. The program reads 4-state character nucleotide data sets and outputs estimates of the three Markov matrices from the root to each taxon. 2STM also calculates the variability of estimates (bootstrap) and some simple statistics, such as composition of nucleotide characters, either in 4 states or 2 states.

The license of this software is Freeware, you can free download and free use this calculator software.